Real time PCR MCQs Welcome to your Real time PCR quiz. 1. Which of the following true about real time PCR? It is an end point quantification method It is a method for real time quantification of the PCR products It is fluorescent probe free method of quantification After real time PCR product can only be detected at the end of the PCR None 2. Real time PCR can be used to detect the nucleic acids present in low concentration. True False None 3. Which of the following condition most essential for the real time PCR based gene expression profiling? Using minimum SYBr green dye for cost saving Conversion of the RNA to cDNA Using fixed number of DNA polymerase None of the above None 4. How primer concentration leads to decrease in PCR efficiency? Primer concentration decreases with time Binding specificity of the primer reduces over cycles Base pairing between the primers reduces their availability Excess template leads to competition for primer None 5. Which of the following method is not the application of real time PCR? Gene copy number detection Determination of the viral load in a sample RNA expression profilling Chromosome number detection None 6. Which of the following enzyme is not used in RNA processing in real time PCR? DNase RNase Reverse transcriptase Lysozyme None 7. What is the role of RNase inhibitor in real time PCR methods? In cDNA synthesis Protection of RNA from degradation by enzyme To stop the spontaneous degradation of the product All of the above None 8. Which of the following is true for traditional and real time PCR? Both the techniques allow detection of the product in early stages Real time PCR can detect the product in early stages as compared to traditional PCR Both systems require different primers Both techniques are end point product determination methods None 9. What is pattern of the graph, PCR product vs time in real time PCR? Exponential Linear Both None None 10. Which of the following influence the PCR efficiency in real time PCR? Increasing PCR product Decreasing primer concentration Temperature Decreased mg I and II None Please fill in the comment box below. Time's up
