Real time PCR MCQs Welcome to your Real time PCR quiz. 1. How primer concentration leads to decrease in PCR efficiency? Primer concentration decreases with time Binding specificity of the primer reduces over cycles Base pairing between the primers reduces their availability Excess template leads to competition for primer None 2. Which of the following enzyme is not used in RNA processing in real time PCR? DNase RNase Reverse transcriptase Lysozyme None 3. Which of the following is important in real time PCR based gene expression study? Primer designing Conversion of RNA to cDNA Isolation of RNA Protecting RNA from degradation All of the above None 4. Which of the following can not be performed using real time PCR? Identification of the tissue type based upon the expression profiling Determination of the viral particle number in the sample Identification of the novel gene All of the above None 5. Which of the following is true for traditional and real time PCR? Both the techniques allow detection of the product in early stages Real time PCR can detect the product in early stages as compared to traditional PCR Both systems require different primers Both techniques are end point product determination methods None 6. Real time PCR can be used to detect the nucleic acids present in low concentration. True False None 7. What is the role of RNase inhibitor in real time PCR methods? In cDNA synthesis Protection of RNA from degradation by enzyme To stop the spontaneous degradation of the product All of the above None 8. Which of the following condition most essential for the real time PCR based gene expression profiling? Using minimum SYBr green dye for cost saving Conversion of the RNA to cDNA Using fixed number of DNA polymerase None of the above None 9. Which of the following method is not the application of real time PCR? Gene copy number detection Determination of the viral load in a sample RNA expression profilling Chromosome number detection None 10. In real time PCR graph, which phase of the graph represent doubling of the PCR product with 100% capacity? Exponential Linear Platue All of them None Please fill in the comment box below. Time's up
