Real time PCR MCQs Welcome to your Real time PCR quiz. 1. How primer concentration leads to decrease in PCR efficiency? Primer concentration decreases with time Binding specificity of the primer reduces over cycles Base pairing between the primers reduces their availability Excess template leads to competition for primer None 2. Which of the following condition most essential for the real time PCR based gene expression profiling? Using minimum SYBr green dye for cost saving Conversion of the RNA to cDNA Using fixed number of DNA polymerase None of the above None 3. What is pattern of the graph, PCR product vs time in real time PCR? Exponential Linear Both None None 4. Which of the following true about real time PCR? It is an end point quantification method It is a method for real time quantification of the PCR products It is fluorescent probe free method of quantification After real time PCR product can only be detected at the end of the PCR None 5. Which of the following technique is not used for gene expression studies? Real time PCR Northern blotting RNase protection assay None of the above None 6. Real time PCR can be used to detect the nucleic acids present in low concentration. True False None 7. Which of the following influence the PCR efficiency in real time PCR? Increasing PCR product Decreasing primer concentration Temperature Decreased mg I and II None 8. In real time PCR graph, which phase of the graph represent doubling of the PCR product with 100% capacity? Exponential Linear Platue All of them None 9. Which of the following can not be performed using real time PCR? Identification of the tissue type based upon the expression profiling Determination of the viral particle number in the sample Identification of the novel gene All of the above None 10. What is the role of RNase inhibitor in real time PCR methods? In cDNA synthesis Protection of RNA from degradation by enzyme To stop the spontaneous degradation of the product All of the above None Please fill in the comment box below. Time's up
