CRISPR Biology Welcome to your CRISPR Biology quiz. Let's get started. 1. CRISPR is best suited for which of the following Random mutagenesis Site specific mutagenesis DNA replacement All of the above None 2. Which of the following gene editing technology was used before CRISPR TALENs ZFNs Restriction digestion and ligation enzyme based gene editing All of the above None 3. Which transformation strategy can be used for CRISPR based gene editing? Through viral vectors Through Transformation Through direct transfer of the nuclease complexes All of the above. None 4. What is PCR? Polyprotein complex regulation Polymerase chain reaction Peripheral cell receptor None of the above None 5. None 6. From which bacteria the CRISPR/Cas9 system has been adopted in plant gene editing? S. pyogenes E. coli Bacteriophage None of the above None 7. What is the function of the guide RNA in CRISPR based gene editing? It guides the Cas9 protein to the target sequence It guides the Cas9 protein to cut the target sequence It helps in the formation of the effector complex It helps in transcription of the Cas9 protein 1 and 2 None 8. Any sequence can be used as a target sequence in CRISPR based gene editing. PAM sequence is essential for targeting PAM specific Cas can only be used for gene targeting The statement is false The statement is true Option 1,2 and 3 None 9. How CRISPR helps in site specific mutagenesis With the help of endonuclease activity and NHEJ With the help of endonuclease activity and HR With the help of endonuclease activity and BER None of the above None 10. Which of the following CRISPR based alteration in DNA is NOT mediated by NHEJ Deletion Insertion Knock out None of the above None Please fill in the comment box below. Time's up
