Basic Biochemistry Welcome to your Basic Biochemistry test. 1. Henderson-hasselbalch equation helps determining pH By using the ratio of the absolute concentrations of the deprotonated to protonated forms of the compound By using log value of the ratio of the absolute concentrations of the deprotonated to protonated forms of the compound By using log of pKa None of the above None 2. Amino acids are linked by Ether bond Phospho-diester bond Peptide bond Glycosidic bond None 3. Paper chromatography separates amino acids based upon Net electrical charge Mass of the molecule 3-D structure of the amino acid pKa values None 4. Charged form of amino acids, Is neutral at pH 7 and this form is called as zwitterion Only COOH is ionised at pH 7 and this form is called as zwitterion Only NH3+ group is ionised at pH7 and this form is called as zwitterion Both the groups are ionised at pH 7 and this form is called as zwitterion None 5. Number of common amino acids in biological system is 20 27 30 11 None 6. Aliphatic groups of the amino acids tend weakly donate the electrons True False None 7. The biological properties of the proteins are conferred by Physical properties of the amino acids Chemical properties of the amino acids Surrounding molecules of the proteins Concentration of the proteins in the cytoplasm 1&2 None 8. Peculiar characteristic of the amino acids is, It can form large number of the conformational variety in the protein structure It acts as acids and also as base It makes protein work under wide range of pH Only 1 1&2 None 9. Which of the following is true about the peptide bond? It is formed only when the alpha carboxyl group reacts with alpha amino group It is formed when any carboxyl group of the amino acid reacts with any amino group of the amino acid One amino acid is part of only one peptide bond Terminal amino acid is a part of two peptide bonds None 10. Proteins are formed of one or mode polypeptides. True False None 11. The difference between the paper electrophoresis and paper chromatography is Electric potential is applied between the two ends of the paper in electrophoresis In paper electrophoresis total charge on the molecule is responsible for the separation In paper chromatography molecules separate on the basis of the partition coefficient All of the above. None 12. Proteins are made of Glucose Amino acids Cholesterol Nucelosides None 13. According to Henderson-hasselbalch equation, in buffers pKa value of the compound is constant pH value is independent of the pKa pH value is dependent upon the -log pKa pH value is nothing but the log pKa None 14. What is pI value? It is a negative log of the pKa value It is a value of pH where amino acids have net zero charge pH at which all the groups in an amino acid are protonated pH at which the proteins have net positive charge at low pH None 15. Partition coefficient is Ability of the amino acid to interact with the molecules of the support material in chromatographic techniques Relative solubility of the amino acids in the polar and non-polar solvents Ability of the amino acids to dissolve in the water Ability of the amino acids to dissolve in non polar solvents None Please fill in the comment box below. Time's up
