Basic Biochemistry Welcome to your Basic Biochemistry test. 1. Amino acids are linked by Ether bond Phospho-diester bond Peptide bond Glycosidic bond None 2. Functionality of the proteins depends on Overall 3-d structure of the protein Chemical properties of the constituent amino acids Intronic sequences All of the above 1 & 2 None 3. Which of the following entity attract electrons from the neighbouring atoms? Amino group Carboxyl group Peptide bond All of the above None 4. Charged form of amino acids, Is neutral at pH 7 and this form is called as zwitterion Only COOH is ionised at pH 7 and this form is called as zwitterion Only NH3+ group is ionised at pH7 and this form is called as zwitterion Both the groups are ionised at pH 7 and this form is called as zwitterion None 5. Amino acid, Has only carboxyl and NH3 functional groups Has only two ionising groups Amino acid side chain can have ionisable functional groups Has -OH as an ionisable group None 6. Which of the following amino acid does not have ionisable side chain? Asp Glu Tyr Lys None 7. Amino acid with three basic functional groups is Lys Arg Glu Asp None 8. Henderson-hasselbalch equation helps determining pH By using the ratio of the absolute concentrations of the deprotonated to protonated forms of the compound By using log value of the ratio of the absolute concentrations of the deprotonated to protonated forms of the compound By using log of pKa None of the above None 9. Peculiar characteristic of the amino acids is, It can form large number of the conformational variety in the protein structure It acts as acids and also as base It makes protein work under wide range of pH Only 1 1&2 None 10. Which of the following is true? pKa values of carboxyl groups in all amino acids are same pKa values of carboxyl groups in the same amino acid are same Neighbouring chemical groups in amino acids influence the pKa value of the group None of the above None 11. Primary structure of the protein is The sequence of the mRNA coding for the corresponding protein The 3-D structure attained by the protein in vitro The amino acid sequence of the polypeptide The sequence of the amino acids that form the active site or binding site of the proteins None 12. Partition coefficient is Ability of the amino acid to interact with the molecules of the support material in chromatographic techniques Relative solubility of the amino acids in the polar and non-polar solvents Ability of the amino acids to dissolve in the water Ability of the amino acids to dissolve in non polar solvents None 13. The biological properties of the proteins are conferred by Physical properties of the amino acids Chemical properties of the amino acids Surrounding molecules of the proteins Concentration of the proteins in the cytoplasm 1&2 None 14. Which of the following is true about the peptide bond? It is formed only when the alpha carboxyl group reacts with alpha amino group It is formed when any carboxyl group of the amino acid reacts with any amino group of the amino acid One amino acid is part of only one peptide bond Terminal amino acid is a part of two peptide bonds None 15. What is pI value? It is a negative log of the pKa value It is a value of pH where amino acids have net zero charge pH at which all the groups in an amino acid are protonated pH at which the proteins have net positive charge at low pH None Please fill in the comment box below. Time's up
