Basic Biochemistry Welcome to your Basic Biochemistry test. 1. Paper chromatography separates amino acids based upon Net electrical charge Mass of the molecule 3-D structure of the amino acid pKa values None 2. Which of the following amino acid does not have ionisable side chain? Asp Glu Tyr Lys None 3. Partition coefficient is Ability of the amino acid to interact with the molecules of the support material in chromatographic techniques Relative solubility of the amino acids in the polar and non-polar solvents Ability of the amino acids to dissolve in the water Ability of the amino acids to dissolve in non polar solvents None 4. The biological properties of the proteins are conferred by Physical properties of the amino acids Chemical properties of the amino acids Surrounding molecules of the proteins Concentration of the proteins in the cytoplasm 1&2 None 5. Peculiar characteristic of the amino acids is, It can form large number of the conformational variety in the protein structure It acts as acids and also as base It makes protein work under wide range of pH Only 1 1&2 None 6. Henderson-hasselbalch equation helps determining pH By using the ratio of the absolute concentrations of the deprotonated to protonated forms of the compound By using log value of the ratio of the absolute concentrations of the deprotonated to protonated forms of the compound By using log of pKa None of the above None 7. Amino acids are linked by Ether bond Phospho-diester bond Peptide bond Glycosidic bond None 8. Number of common amino acids in biological system is 20 27 30 11 None 9. Primary structure of the protein is The sequence of the mRNA coding for the corresponding protein The 3-D structure attained by the protein in vitro The amino acid sequence of the polypeptide The sequence of the amino acids that form the active site or binding site of the proteins None 10. Amino acid, Has only carboxyl and NH3 functional groups Has only two ionising groups Amino acid side chain can have ionisable functional groups Has -OH as an ionisable group None 11. What is pI value? It is a negative log of the pKa value It is a value of pH where amino acids have net zero charge pH at which all the groups in an amino acid are protonated pH at which the proteins have net positive charge at low pH None 12. Proteins are made of Glucose Amino acids Cholesterol Nucelosides None 13. Aliphatic groups of the amino acids tend weakly donate the electrons True False None 14. Which of the following entity attract electrons from the neighbouring atoms? Amino group Carboxyl group Peptide bond All of the above None 15. Proteins are formed of one or mode polypeptides. True False None Please fill in the comment box below. Time's up
