Basic Biochemistry Welcome to your Basic Biochemistry test. 1. According to Henderson-hasselbalch equation, in buffers pKa value of the compound is constant pH value is independent of the pKa pH value is dependent upon the -log pKa pH value is nothing but the log pKa None 2. Functionality of the proteins depends on Overall 3-d structure of the protein Chemical properties of the constituent amino acids Intronic sequences All of the above 1 & 2 None 3. Which of the following amino acid is most commonly involved in the disulphide bond formation? Cys Asp Ser Glu None 4. Primary structure of the protein is The sequence of the mRNA coding for the corresponding protein The 3-D structure attained by the protein in vitro The amino acid sequence of the polypeptide The sequence of the amino acids that form the active site or binding site of the proteins None 5. Amino acid, Has only carboxyl and NH3 functional groups Has only two ionising groups Amino acid side chain can have ionisable functional groups Has -OH as an ionisable group None 6. Partition coefficient is Ability of the amino acid to interact with the molecules of the support material in chromatographic techniques Relative solubility of the amino acids in the polar and non-polar solvents Ability of the amino acids to dissolve in the water Ability of the amino acids to dissolve in non polar solvents None 7. Which of the following amino acid does not have ionisable side chain? Asp Glu Tyr Lys None 8. Which of the following is true about the peptide bond? It is formed only when the alpha carboxyl group reacts with alpha amino group It is formed when any carboxyl group of the amino acid reacts with any amino group of the amino acid One amino acid is part of only one peptide bond Terminal amino acid is a part of two peptide bonds None 9. The biological properties of the proteins are conferred by Physical properties of the amino acids Chemical properties of the amino acids Surrounding molecules of the proteins Concentration of the proteins in the cytoplasm 1&2 None 10. Amino acids are linked by Ether bond Phospho-diester bond Peptide bond Glycosidic bond None 11. Aliphatic groups of the amino acids tend weakly donate the electrons True False None 12. Peculiar characteristic of the amino acids is, It can form large number of the conformational variety in the protein structure It acts as acids and also as base It makes protein work under wide range of pH Only 1 1&2 None 13. Proteins are formed of one or mode polypeptides. True False None 14. Number of common amino acids in biological system is 20 27 30 11 None 15. Paper chromatography separates amino acids based upon Net electrical charge Mass of the molecule 3-D structure of the amino acid pKa values None Please fill in the comment box below. Time's up
