Basic Biochemistry Welcome to your Basic Biochemistry test. 1. Henderson-hasselbalch equation helps determining pH By using the ratio of the absolute concentrations of the deprotonated to protonated forms of the compound By using log value of the ratio of the absolute concentrations of the deprotonated to protonated forms of the compound By using log of pKa None of the above None 2. Functionality of the proteins depends on Overall 3-d structure of the protein Chemical properties of the constituent amino acids Intronic sequences All of the above 1 & 2 None 3. Aliphatic groups of the amino acids tend weakly donate the electrons True False None 4. Which of the following is true about the peptide bond? It is formed only when the alpha carboxyl group reacts with alpha amino group It is formed when any carboxyl group of the amino acid reacts with any amino group of the amino acid One amino acid is part of only one peptide bond Terminal amino acid is a part of two peptide bonds None 5. Primary structure of the protein is The sequence of the mRNA coding for the corresponding protein The 3-D structure attained by the protein in vitro The amino acid sequence of the polypeptide The sequence of the amino acids that form the active site or binding site of the proteins None 6. What is pI value? It is a negative log of the pKa value It is a value of pH where amino acids have net zero charge pH at which all the groups in an amino acid are protonated pH at which the proteins have net positive charge at low pH None 7. Amino acids are linked by Ether bond Phospho-diester bond Peptide bond Glycosidic bond None 8. Paper chromatography separates amino acids based upon Net electrical charge Mass of the molecule 3-D structure of the amino acid pKa values None 9. Which of the following is true? pKa values of carboxyl groups in all amino acids are same pKa values of carboxyl groups in the same amino acid are same Neighbouring chemical groups in amino acids influence the pKa value of the group None of the above None 10. The difference between the paper electrophoresis and paper chromatography is Electric potential is applied between the two ends of the paper in electrophoresis In paper electrophoresis total charge on the molecule is responsible for the separation In paper chromatography molecules separate on the basis of the partition coefficient All of the above. None 11. Proteins are made of Glucose Amino acids Cholesterol Nucelosides None 12. Proteins are formed of one or mode polypeptides. True False None 13. Partition coefficient is Ability of the amino acid to interact with the molecules of the support material in chromatographic techniques Relative solubility of the amino acids in the polar and non-polar solvents Ability of the amino acids to dissolve in the water Ability of the amino acids to dissolve in non polar solvents None 14. The biological properties of the proteins are conferred by Physical properties of the amino acids Chemical properties of the amino acids Surrounding molecules of the proteins Concentration of the proteins in the cytoplasm 1&2 None 15. Number of common amino acids in biological system is 20 27 30 11 None Please fill in the comment box below. Time's up
