Basic Biochemistry Welcome to your Basic Biochemistry test. 1. Which of the following amino acid does not have ionisable side chain? Asp Glu Tyr Lys None 2. Aliphatic groups of the amino acids tend weakly donate the electrons True False None 3. Amino acid, Has only carboxyl and NH3 functional groups Has only two ionising groups Amino acid side chain can have ionisable functional groups Has -OH as an ionisable group None 4. The difference between the paper electrophoresis and paper chromatography is Electric potential is applied between the two ends of the paper in electrophoresis In paper electrophoresis total charge on the molecule is responsible for the separation In paper chromatography molecules separate on the basis of the partition coefficient All of the above. None 5. Peculiar characteristic of the amino acids is, It can form large number of the conformational variety in the protein structure It acts as acids and also as base It makes protein work under wide range of pH Only 1 1&2 None 6. Functionality of the proteins depends on Overall 3-d structure of the protein Chemical properties of the constituent amino acids Intronic sequences All of the above 1 & 2 None 7. Primary structure of the protein is The sequence of the mRNA coding for the corresponding protein The 3-D structure attained by the protein in vitro The amino acid sequence of the polypeptide The sequence of the amino acids that form the active site or binding site of the proteins None 8. Partition coefficient is Ability of the amino acid to interact with the molecules of the support material in chromatographic techniques Relative solubility of the amino acids in the polar and non-polar solvents Ability of the amino acids to dissolve in the water Ability of the amino acids to dissolve in non polar solvents None 9. Amino acids are linked by Ether bond Phospho-diester bond Peptide bond Glycosidic bond None 10. Proteins are formed of one or mode polypeptides. True False None 11. Henderson-hasselbalch equation helps determining pH By using the ratio of the absolute concentrations of the deprotonated to protonated forms of the compound By using log value of the ratio of the absolute concentrations of the deprotonated to protonated forms of the compound By using log of pKa None of the above None 12. Which of the following is true about the peptide bond? It is formed only when the alpha carboxyl group reacts with alpha amino group It is formed when any carboxyl group of the amino acid reacts with any amino group of the amino acid One amino acid is part of only one peptide bond Terminal amino acid is a part of two peptide bonds None 13. According to Henderson-hasselbalch equation, in buffers pKa value of the compound is constant pH value is independent of the pKa pH value is dependent upon the -log pKa pH value is nothing but the log pKa None 14. Which of the following amino acid is most commonly involved in the disulphide bond formation? Cys Asp Ser Glu None 15. The biological properties of the proteins are conferred by Physical properties of the amino acids Chemical properties of the amino acids Surrounding molecules of the proteins Concentration of the proteins in the cytoplasm 1&2 None Please fill in the comment box below. Time's up
